MCCCD Program Description

Maintaining Biological Laboratories

Course: FAC250

First Term: 2007 Spring

Lecture 3 Credit(s) 3 Period(s) 3 Load
Subject Type: Occupational
Load Formula: S

Description: Standards and guidelines for Biosafety Level (BSL 1-4) ventilation and exhaust of air. Types of filtration and control associated with the four levels of biological research laboratories

MCCCD Official Course Competencies
1. Differentiate the Biosafety Level (BSL 1 - 4) for laboratories Criteria. (I) 2. Differentiate the ventilation requirements for the four types of (BSL 1-4). (II) 3. Differentiate the filtration incoming and exhaust requirements for the four types of (BSL 1-4). (III) 4. Differentiate the requirements for class II biosafety cabinets and the four types: A1, A2, B1, and B2 based on primary air flow and percent of exhaust. (IV) 5. Evaluate specific standards and guidelines to level 3 biological research laboratory. (V) 6. Evaluate specific airflow requirements based on guidelines applying to biological safety cabinets. (VI) 7. Compare and contrast the following designs for laboratory airflow: constant volume (CV), two-state, variable air volume (VAV), and usage based controls (UBC). (VII)
MCCCD Official Course Competencies must be coordinated with the content outline so that each major point in the outline serves one or more competencies. MCCCD faculty retains authority in determining the pedagogical approach, methodology, content sequencing, and assessment metrics for student work. Please see individual course syllabi for additional information, including specific course requirements.

MCCCD Official Course Outline
I. Laboratory Biosafety Level Criteria 1. Biosafety level 1 (BSL-1) 2. Biosafety level 2 (BSL-2) 3. Biosafety level 3 (BSL-3) 4. Biosafety level 4 (BSL-4) II. Ventilation 1. Biosafety level 1 (BSL-1) 2. Biosafety level 2 (BSL-2) 3. Biosafety level 3 (BSL-3) 4. Biosafety level 4 (BSL-4) III. Infiltration and Exhaust 1. Biosafety level 1 (BSL-1) 2. Biosafety level 2 (BSL-2) 3. Biosafety level 3 (BSL-3) 4. Biosafety level 4 (BSL-4) IV. Biosafety Cabinets 1. Biosafety level 1 (BSL-1) 2. Biosafety level 2 (BSL-2) 3. Biosafety level 3 (BSL-3) 4. Biosafety level 4 (BSL-4) V. Standards and Guidelines 1. Centers for Disease Control and Prevention (CDC) and National Institute of health (NIH) 2. National Science Foundation (NSF)/American National Science Institute (ANSI) 49-2002 Class II (laminar flow) biosafety cabinetry. NSF International Standard/American national Standard 2002 3. Health Canada, laboratory Biosafety Guidelines, Third edition 4. United States Department of Agriculture (USDA), Agricultural Research services (ARS), ARS Facilities Design Standard (2002) 5. American Society of Heating, Refrigerating and Air- Conditioning Engineers (ASHRAE) Handbook 1999 HVAC Applications, Chapter 13, laboratories 6. Guidelines listed in Phoenix Controls BSL-3 Laboratory Design Resource VI. Air Flow Requirements 1. Biosafety level 1 (BSL-1) 2. Biosafety level 2 (BSL-2) 3. Biosafety level 3 (BSL-3) 4. Biosafety level 4 (BSL-4) VII. Air Flow Designs and Controls 1. Constant Volume 2. Two-state 3. Variable Air Volume 4. Control of 1-3

MCCCD Governing Board Approval Date: 12/12/2006

All information published is subject to change without notice. Every effort has been made to ensure the accuracy of information presented, but based on the dynamic nature of the curricular process, course and program information is subject to change in order to reflect the most current information available.