MCCCD Program Description

Advanced Lab Instrumentation

Course: BIO210

First Term: 2018 Fall

Lec + Lab 3 Credit(s) 6 Period(s) 6 Load
Subject Type: Academic
Load Formula: T Lab Load

Description: Provides training in the principles, operation and interpretation of data from laboratory instrumentation.

MCCCD Official Course Competencies
1. Demonstrate an understanding of safe and accepted practices in the laboratory including Good Laboratory Practice (GLP). (I) 2. Apply standard lab operating procedures in sampling, sample handling, and sample preparation. (II). 3. Describe the statistics of sampling. (I, II) 4. Present evidence of effective record keeping in the lab. (I). 5. Explain the fundamental concepts of instrumental analysis. (III) 6. Describe the common characteristics underlying instruments that rely on spectrochemical methods. (IV) 7. Measure sample absorbance with UV-VIS spectrometry. (IV, V) 8. Explain the specific advantages and limitations of techniques in IR spectrometry and fluorometry. (V). 9. Describe the applications and procedural steps of Atomic spectroscopy and X-ray diffraction spectroscopy. (VI) 10. Demonstrate a procedural understanding of nuclear magnetic resonance. (VI) 11. Explain the principles and instrument design involved with mass spectrometry (VI) 12. Execute analytical separations involving gas chromatography and high-performance liquid chromatography (HPLC). (VII, VIII) 13. Interpret data derived from a nuclear magnetic resonance spectrometer, a mass spectrometer, a gas chromatograph and a HPLC instrument. (VI, VII, VIII)
MCCCD Official Course Competencies must be coordinated with the content outline so that each major point in the outline serves one or more competencies. MCCCD faculty retains authority in determining the pedagogical approach, methodology, content sequencing, and assessment metrics for student work. Please see individual course syllabi for additional information, including specific course requirements.

MCCCD Official Course Outline
I. Fundamental Laboratory practices A. Laboratory Protocol B. MSDS forms C. Reading container labels D. Proper storage and disposal of chemicals E. Laboratory notebook F. Good Laboratory Practices (GLP) G. Errors, statistics and statistical control II. Sampling and sample preparation and storage A. Statistics of sampling B. Sample handling C. Sample preparation D. Sample stability III. Introduction to Instrumental Analysis A. Basics of instrumental measurement B. Preparation of standards and controls IV. Introduction to Spectrochemical Methods A. Characteristics of the electromagnetic spectrum B. Absorption and emission of light C. Absorbance, transmittance and Beer`s Law V. UV-VIS and IR Molecular Spectrometry A. UV-VIS instrumentation B. Fluorometry C. Introduction to IR spectrometry D. Basic IR spectra interpretation VI. Other Spectroscopic Methods A. Atomic Spectroscopy B. Nuclear Magnetic Resonance Spectrometry (NMR) C. Basic interpretation of NMR spectra D. Theory and practice of Mass Spectrometry (MS) E. MS instrument design:Quadrupole and Time of Flight F. Basic MS spectra interpretation VII. Gas Chromatography (GC) A. Techniques of Gas Chromatography B. Instrument Design C. Sample injection D. Columns E. GC Detectors:Thermal conductivity and Flame Ionization F. Adaptations of the GC technique:GC-MS and GC-IR VIII. High-Performance Liquid Chromatography (HPLC) A. Techniques of HPLC B. Instrument Design C. Mobile phases D. Gradient or isocratic elution E. Sample injection F. Columns G. Detectors:UV absorption and Diode Array H. Adaptations of the HPLC technique:LS-MS and LC-IR

MCCCD Governing Board Approval Date: June 28, 2016

All information published is subject to change without notice. Every effort has been made to ensure the accuracy of information presented, but based on the dynamic nature of the curricular process, course and program information is subject to change in order to reflect the most current information available.