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Center for Curriculum and Transfer Articulation
Biomolecular Separation
Course: BIO209

First Term: 2018 Fall
 Lec + Lab   3.0 Credit(s)   6.0 Period(s)   6.0 Load  
Subject Type: Academic
Load Formula: T Lab Load


Description: Introduction to industrial laboratory biotechnology with intensive focus on the recovery of heterologous proteins and other biomolecules from cultivated cells and the subsequent recovery, purification and characterization of these molecules.



MCCCD Official Course Competencies
1. Describe growth characteristics of bacterial, yeast and plant cells. (I)
2. Describe conditions required for normal cell growth. (I)
3. Apply biological concepts to grow bacterial, yeast and plant cells in culture. (I, II)
4. Operate and troubleshoot apparatus used for cell culture. (II)
5. Prepare and maintain viable cell cultures from various sources. (II, III)
6. Use appropriate techniques to introduce nucleic acids into cells and monitor their expression. (IV)
7. Separate synthesized proteins and other biomolecules using appropriate isolation techniques and check for purity and concentration. (IV)
MCCCD Official Course Competencies must be coordinated with the content outline so that each major point in the outline serves one or more competencies. MCCCD faculty retains authority in determining the pedagogical approach, methodology, content sequencing, and assessment metrics for student work. Please see individual course syllabi for additional information, including specific course requirements.
 
MCCCD Official Course Outline
I. Cell growth and culture
      A. Introduction to cell metabolism
      B. Growth characteristics of bacterial, yeast and plant cells
      C. Conditions required for normal cell growth
II. Laboratory and industrial applications
      A. Design, operation, monitoring and maintenance of equipment
      B. Physical and physiological requirements for cell growth
            1. Nutrients, media and lighting levels
            2. pH
            3. Dissolved oxygen and carbon dioxide concentrations
            4. Osmolality and conductivity
      C. Quality control and compliance
III. Cell and tissue culture techniques
      A. Media preparation and maintenance
      B. Sterile technique
IV. Use of cell culture to express heterologous deoxyribonucleic acid (DNA) sequences
      A. Introduction of nucleic acid sequences to form recombinant cells
            1. Transformation
            2. Electroporation
            3. Other
      B. Cell lysis
            1. Enzymatic
            2. Sonication
      C. Characterization, purification and quantitation techniques from cellular homogenates or media
            1. Centrifugation
            2. Chromatography
            3. Filtration
            4. Gel electrophoresis
            5. Spectrophotometry
            6. Antibody detection
            7. Enzyme assays
      D. Scaling up production for industrial applications
 
MCCCD Governing Board Approval Date: June 28, 2016

All information published is subject to change without notice. Every effort has been made to ensure the accuracy of information presented, but based on the dynamic nature of the curricular process, course and program information is subject to change in order to reflect the most current information available.